Combining signals from spotted cDNA microarrays 1 obtained at different scanning intensities
نویسنده
چکیده
3 H. P. Piepho* 4 5 Bioinformatics Unit, Institute for Crop Production and Grassland Research, University 6 of Hohenheim, Fruwirthstr. 23, 70599 Stuttgart, Germany 7 8 B. Keller 9 10 Bioinformatics Unit, Institute for Crop Production and Grassland Research, University 11 of Hohenheim, Fruwirthstr. 23, 70599 Stuttgart, Germany 12 13 N. Hoecker 14 15 Center for Plant Molecular Biology, Department of General Genetics, University of 16 Tuebingen, Auf der Morgenstelle 28, 72076 Tuebingen, Germany 17 18 F. Hochholdinger 19 20 Center for Plant Molecular Biology, Department of General Genetics, University of 21 Tuebingen, Auf der Morgenstelle 28, 72076 Tuebingen, Germany 22 23 24 *To whom correspondence should be sent 25 © The Author (2006). Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected]
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Combining signals from spotted cDNA microarrays obtained at different scanning intensities
MOTIVATION The analysis of spotted cDNA microarrays involves scanning of color signals from fluorescent dyes. A common problem is that a given scanning intensity is not usually optimal for all spotted cDNAs. Specifically, some spots may be at the saturation limit, resulting in poor separation of signals from different tissues or conditions. The problem may be addressed by multiple scans with va...
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